SERS assessment of the cancer-specific methylation pattern of genomic DNA: towards the detection of acute myeloid leukemia in patients undergoing hematopoietic stem cell transplantation

Abstract

In this label-free surface-enhanced Raman scattering (SERS) study of genomic DNA, we demonstrate that the cancer-specific DNA methylation pattern translates into specific spectral differences. Thus, DNA extracted from an acute myeloid leukemia (AML) cell line presented a decreased intensity of the 1005 cm−1 band of 5-methylcytosine compared to normal DNA, in line with the well-described hypomethylation of cancer DNA. The unique methylation pattern of cancer DNA also influences the DNA adsorption geometry, resulting in higher adenine SERS intensities for cancer DNA. The possibility of detecting cancer DNA based on its SERS spectrum was validated on peripheral blood genomic DNA samples from n = 17 AML patients and n = 17 control samples, yielding an overall classification of 82% based on the 1005 cm−1 band of 5-methylcytosine. By demonstrating the potential of SERS in assessing the methylation status in the case of real-life DNA samples, the study paves the way for novel methods of diagnosing cancer.

Authors

Vlad Moisoiu
(Faculty of Physics, Babeș-Bolyai University, Cluj-Napoca, Romania
Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania)
Andrei Stefancu
(Faculty of Physics, Babeș-Bolyai University, Cluj-Napoca, Romania)
Stefania D. Iancu
(Faculty of Physics, Babeș-Bolyai University, Cluj-Napoca, Romania)
Tudor Moisoiu
(Clinical Institute of Urology and Renal Transplant, Cluj-Napoca, Romania)
Luminita Loga
(Clinical Institute of Urology and Renal Transplant, Cluj-Napoca, Romania)
Lucia Dican
(Department of Biochemistry, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania)
Cristian D. Alecsa
(Tiberiu Popoviciu Institute of Numerical Analysis, Cluj-Napoca, Romania)
Imre Boros
(Tiberiu Popoviciu Institute of Numerical Analysis, Cluj-Napoca, Romania)
Anca Jurj
(Research Center for Functional Genomics and Translational Medicine/Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania)
Delia Dima
(Department of Hematology, Ion Chiricuta Clinical Cancer Center, Cluj-Napoca, Romania)
Cristina Bagacean
(INSERM UMR1227, B Lymphocytes and Autoimmunity, University Bretagne Occidentale, Brest, France)
Romulus Tetean
(Faculty of Physics, Babeș-Bolyai University, Cluj-Napoca, Romania)
Emil Burzo
(Faculty of Physics, Babeș-Bolyai University, Cluj-Napoca, Romania)
Ciprian Tomuleasa
(Research Center for Functional Genomics and Translational Medicine/Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania,
Department of Hematology, Ion Chiricuta Clinical Cancer Center, Cluj-Napoca, Romania
Department of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania)
Florin Elec
(Clinical Institute of Urology and Renal Transplant, Cluj-Napoca, Romania,
Department of Urology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania)
Nicolae Leopold
(Faculty of Physics, Babeș-Bolyai University, Cluj-Napoca, Romania)

Keywords

Acute myeloid leukemia; 5-Methylcytosine; surface-enhanced Raman scattering (SERS); DNA methylation; Epigenetics 

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Paper coordinates

V. Moisoiu, A. Stefancu, S.D. Iancu, T. Moisoiu, L. Loga, L. Dican, C.D. Alecsa, I. Boros, A. Jurj, D. Dima, C. Bagacean, R. Tetean, E. Burzo, C. Tomuleasa, F. Elec, N. Leopold, SERS assessment of the cancer-specific methylation pattern of genomic DNA: towards the detection of acute myeloid leukemia in patients undergoing hematopoietic stem cell transplantation, Analytical and Bioanalytical Chemistry, 411 (2019) no. 29, pp. 7907–7913.
DOi: 10.1007/s00216-019-02213-2

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Analytical and Bioanalytical Chemistry

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Springer

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References

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